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any of a large variety of proteins normally present in the body or produced in response to an antigen which it neutralizes, thus producing an immune response

PrepTutorEJDIC

抗体,免疫体,抗毒素

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1. 赤痢アメーバによる腸菅アメーバ症 intestinal entamoeba histolytica amebiasis
2. 赤痢アメーバによる腸菅外アメーバ症 extraintestinal entamoeba histolytica amebiasis
3. 資源に富んだ国における急性下痢の疫学および原因 epidemiology and causes of acute diarrhea in resource rich countries
4. 脳膿瘍の病因、臨床症状、および診断 pathogenesis clinical manifestations and diagnosis of brain abscess
5. 原発性免疫不全の胃腸症状 gastrointestinal manifestations in primary immunodeficiency

English Journal

Nano-yeast-scFv probes on screen-printed gold electrodes for detection of Entamoeba histolytica antigens in a biological matrix.

Grewal YS1, Shiddiky MJ2, Spadafora LJ3, Cangelosi GA3, Trau M4.Author information 1Centre for Biomarker Research and Development, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane QLD 4072, Australia.2Centre for Biomarker Research and Development, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane QLD 4072, Australia. Electronic address: m.shiddiky@uq.edu.au.3School of Public Health University of Washington, Seattle, WA, USA.4Centre for Biomarker Research and Development, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane QLD 4072, Australia; School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane QLD 4072, Australia. Electronic address: m.trau@uq.edu.au.AbstractThe time and costs associated with monoclonal antibody production limit the potential for portable diagnostic devices to penetrate the market. Replacing the antibody with a low-cost alternate affinity reagent would reduce the costs of diagnostic development and use, and lead to new portable diagnostic devices towards many diseases. Herein, we present low-cost affinity reagents, nano-yeast-scFv, on commercially available, inexpensive, and portable screen-printed electrodes for the label-free electrochemical detection of Entamoeba histolytica cyst antigens. The biosensor was able to detect antigen at concentrations down to 10pgmL(-1) in buffer with an inter-assay reproducibility of (% RSD, n=3) 4.1%. The applicability of two differently engineered nano-yeast-scFv to each specifically detect their cognant E. histolytica cyst antigens was demonstrated in a biological matrix derived from human stool. Because of the simple, inexpensive, and sensitive nature of this methodology, it may offer a low-cost alternative to immunosensors based on antibody-target recognition.
Biosensors & bioelectronics.Biosens Bioelectron.2014 May 15;55:417-22. doi: 10.1016/j.bios.2013.12.043. Epub 2013 Dec 27.
The time and costs associated with monoclonal antibody production limit the potential for portable diagnostic devices to penetrate the market. Replacing the antibody with a low-cost alternate affinity reagent would reduce the costs of diagnostic development and use, and lead to new portable diagnost
PMID 24434498

Clinical Significance of High Anti-Entamoeba histolytica Antibody Titer in Asymptomatic HIV-1-infected Individuals.

Watanabe K, Aoki T, Nagata N, Tanuma J, Kikuchi Y, Oka S, Gatanaga H.Author information AIDS Clinical Center, National Center for Global Health and Medicine, Tokyo, Japan.AbstractBackground. Anti-Entamoeba histolytica antibody (anti- E. histolytica) is widely used in seroprevalence studies though its clinical significance has not been assessed previously.Methods. Anti-E. histolytica titer was measured at first visit to our clinic (baseline) in 1303 patients infected with human immunodeficiency virus type 1 (HIV-1). The time to diagnosis of invasive amebiasis was assessed by Kaplan-Meier method and risk factors for the development of invasive amebiasis were assessed by Cox proportional-hazards regression analysis. For patients who developed invasive amebiasis, anti-E. histolytica titers at onset were compared with those at baseline and after treatment.Results. The anti-E. histolytica seroprevalence in the study population was 21.3% (277/1303). Eighteen patients developed invasive amebiasis during the treatment-free period among 1207 patients who had no history of previous treatment with nitroimidazole. Patients with high anti-E. histolytica titer at baseline developed invasive amebiasis more frequently than those with low anti-E. histolytica titer. Most cases of invasive amebiasis who had high anti-E. histolytica titer at baseline developed within 1 year. High anti-E. histolytica titer was the only independent predictor of future invasive amebiasis. Anti-E. histolytica titer was elevated at the onset of invasive amebiasis in patients with low anti-E. histolytica titer at baseline.Conclusions. Asymptomatic HIV-1-infected individuals with high anti-E. histolytica titer are at risk of invasive amebiasis probably due to exacerbation of subclinical amebiasis.
The Journal of infectious diseases.J Infect Dis.2014 Jan 2. [Epub ahead of print]
Background. Anti-Entamoeba histolytica antibody (anti- E. histolytica) is widely used in seroprevalence studies though its clinical significance has not been assessed previously.Methods. Anti-E. histolytica titer was measured at first visit to our clinic (baseline) in 1303 patients infected with
PMID 24338349

Entamoeba histolytica-secreted cysteine proteases induce IL-8 production in human mast cells via a PAR2-independent mechanism.

Lee YA1, Nam YH1, Min A1, Kim KA1, Nozaki T2, Saito-Nakano Y2, Mirelman D3, Shin MH1.Author information 1Department of Environmental Medical Biology, Institute of Tropical Medicine, and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Korea.2Department of Parasitology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, Japan.3Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel.AbstractEntamoeba histolytica is an extracellular tissue parasite causing colitis and occasional liver abscess in humans. E. histolytica-derived secretory products (SPs) contain large amounts of cysteine proteases (CPs), one of the important amoebic virulence factors. Although tissue-residing mast cells play an important role in the mucosal inflammatory response to this pathogen, it is not known whether the SPs induce mast cell activation. In this study, when human mast cells (HMC-1 cells) were stimulated with SPs collected from pathogenic wild-type amoebae, interleukin IL-8 mRNA expression and production were significantly increased compared with cells incubated with medium alone. Inhibition of CP activity in the SPs with heat or the CP inhibitor E64 resulted in significant reduction of IL-8 production. Moreover, SPs obtained from inhibitors of cysteine protease (ICP)-overexpressing amoebae with low CP activity showed weaker stimulatory effects on IL-8 production than the wild-type control. Preincubation of HMC-1 cells with antibodies to human protease-activated receptor 2 (PAR2) did not affect the SP-induced IL-8 production. These results suggest that cysteine proteases in E. histolytica-derived secretory products stimulate mast cells to produce IL-8 via a PAR2-independent mechanism, which contributes to IL-8-mediated tissue inflammatory responses during the early phase of human amoebiasis.
Parasite (Paris, France).Parasite.2014;21:1. Epub 2014 Feb 7.
Entamoeba histolytica is an extracellular tissue parasite causing colitis and occasional liver abscess in humans. E. histolytica-derived secretory products (SPs) contain large amounts of cysteine proteases (CPs), one of the important amoebic virulence factors. Although tissue-residing mast cells pla
PMID 24502918

Japanese Journal

胃に穿破したアメーバ性肝膿瘍に対し内服治療のみで根治を得た1例

高橋憲史,荒川和久,須納瀬豊,戸塚統,戸谷裕之,竹吉泉
日本消化器病學會雜誌 = The Japanese journal of gastro-enterology 108(8), 1413-1419, 2011-08-05
症例は26歳，男性．肝腫瘍疑いで近医より当院紹介となった．腹部CTで肝左葉に8×6cmの腫瘤を認め，肝膿瘍を疑った．上部消化管内視鏡で胃小彎に巨大な粘膜下の隆起があり，隆起上に膿汁が流出する潰瘍を認め，膿瘍の穿破と判断した．潰瘍の生検でアメーバ虫体が確認された．膿瘍のドレナージは行わず，メトロニダゾールの内服のみで治癒し得た．胃に穿破したアメーバ性肝膿瘍はまれな疾患であり，文献的考察を加え報告する …
NAID 10030298139

細胞診が診断に有用であったアメーバ性肝膿瘍の1例

三谷美湖,高橋保,森木利昭,植田庄介,一圓美穂,根本禎久,熊澤秀雄
日本臨床細胞学会雑誌 42(1), 45-48, 2003-01-22
NAID 110001222455

男性同性愛者にみられたアメーバ性肝膿瘍の1例

瀧朋子,今津博雄,宮本洋二,野口隆一,小林洋三,中谷敏也,中山雅樹,松井勉,福井博,吉川正英,石坂重昭
Journal of Nara Medical Association 52(1), 24-28, 2001-02-28
… The clinical diagnosis of amebiasis was established by the positive antibody of Entamoeba'histolytica. …
NAID 120004973133