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a chemical agent for use in chemical reactions
German bacteriologist who found a `magic bullet to cure syphilis and was a pioneer in the study of immunology (1854-1915) (同)Paul_Ehrlich

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2015/07/23 09:05:00」(JST)

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p-DMAB: the active ingredient in Ehrlich's reagent

Ehrlich's reagent, also known as the "DMAB test", is a chemical test to presumptively identify indoles. It is primarily used as a simple spot-test to identify possible psychoactive compounds such as tryptamines (e.g. DMT) and ergoloids (e.g. LSD). The reagent will also give a positive result for opium, despite the active alkaloids (opiates) not containing the indole moiety, because of the presence in opium of tryptophan.^[1]

The reagent is prepared by dissolving 0.5^[2]-2.0 g of p–dimethylaminobenzaldehyde (DMAB) in 50 mL of 95% ethanol and 50 mL of concentrated hydrochloric acid^[3]^[4] and is best used when fresh.

Other alcohols such as 1-Propanol can also be used to prepare the ehrlich reagent.^[5]

The ehrlich reagent is similar to a number of other indole tests:

The van Urk reagent which uses DMAB, sulphuric acid and an oxidant.^[6]
The Renz and Loew reagent which uses p-dimethylaminocinnamaldehyde and may also be used for the detection of flavonoids.
The "improved hallucinogen reagent" which uses 5 g DMAB in 100 mL concentrated phosphoric acid (specific gravity 1.75) and 100 mL of methanol.^[7]^[1]

References

^ ^a ^b de Faubert Maunder, MJ. "Field and laboratory test for raw and prepared opium.". Bulletin on narcotics 27 (1): 71–6. PMID 1039285.
^ Spratley, Trinette (2004). "Analytical Profiles for Five "Designer" Tryptamines" (PDF). Microgram Journal 3 (1-2): 55. Retrieved 2013-10-09.
^ O’Neal, Carol L; Crouch, Dennis J; Fatah, Alim A (April 2000). "Validation of twelve chemical spot tests for the detection of drugs of abuse". Forensic Science International 109 (3): 189–201. doi:10.1016/S0379-0738(99)00235-2.
^ "Color Test Reagents/Kits for Preliminary Identification of Drugs of Abuse" (PDF). Law Enforcement and Corrections Standards and Testing Program. July 2000. Retrieved 2011-07-24.
^ 02 July 2014. "Ehrlich's Reagent Safety Data Sheet" (PDF). Labchem. Retrieved 11 January 2015.
^ Ehmann, A. (1977). "The van URK-Salkowski reagent — a sensitive and specific chromogenic reagent for silica gel thin-layer chromatographic detection and identification of indole derivatives" (PDF). Journal of Chromatography A 132 (2): 267–201. doi:10.1016/S0021-9673(00)89300-0. edit
^ Maunder, M. J. de Faubert (August 1974). "A field test for hallucinogens: further improvements". Journal of Pharmacy and Pharmacology 26 (8): 637–638. doi:10.1111/j.2042-7158.1974.tb10677.x.

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English Journal

Product in indole detection by Ehrlich's reagent.

Lamb AC1, Federico-Perez RA1, Xue ZL2.
Analytical biochemistry.Anal Biochem.2015 Sep 1;484:21-3. doi: 10.1016/j.ab.2015.04.033. Epub 2015 May 7.
Ehrlich's reagent (p-dimethylaminobenzaldehyde [DMAB, 1] in 95% EtOH with HCl as catalyst) was employed in spot tests of indoles, providing a diagnosis of, for example, liver diseases, hemolytic processes, occlusion of the common bile duct, and carcinoid syndrome. Although the reagent has been widel
PMID 25958008

Synthesis, characterization and biological activity of 2-acetylpyridine-α-naphthoxyacetylhydrazone its metal complexes.

El-Gammal OA1, Bekheit MM2, Tahoon M2.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.Spectrochim Acta A Mol Biomol Spectrosc.2015 Jan 25;135:597-607. doi: 10.1016/j.saa.2014.05.071. Epub 2014 Jul 3.
A new series of complexes of Ni(II), Co(II), Cu(II), Cd(II), Mn(II), Hg(II) and UO2(2+) derived from 2-acetylpyridine-α-naphthoxyacetylhydrazone (HA2PNA) have been prepared and characterized by elemental analyses, spectral (IR, UV-visible, ESR and (1)H NMR) as well as magnetic and thermal measureme
PMID 25124845

[Therapeutic activity of gemcitabine in intracranial tumors].

Stukov AN, Filatova LV, Latipova DKh, Bespalov VG, Belyaeva OA, Kireeva GS, Vasilieva IN, Alexandrov VA, Maidin MA, Semenov AL, Vershinina SF, Markochev AB, Abduloeva NKh, Chubenko VA, Semiglazova TY.
Voprosy onkologii.Vopr Onkol.2015;61(2):274-9.
Gemcitabine is known to exert a therapeutic effect on brain tumors despite the limited permeability of the blood-brain barrier (BBB). In our experimental research single intraperitoneal (i.p.) injection of gemcitabine 25 mg/kg provided increase in median survival of mice with intracranially transpla
PMID 26087611

Japanese Journal

指定薬物の分析Part II : 呈色試験及びTLC

内山奈穂子,河村麻衣子,鎌倉浩之 [他],花尻(木倉) 瑠理,合田幸広
藥學雜誌 128(6), 981-987, 2008-06-01
… The color tests were examined with the Marquis, Ehrlich, Simon's, Liebermann-Burehard's, and Mandelin reagents. … The color of β-carbonyl-methylenedioxyphenetylamines produced by the Marquis reagent was yellow, and 4-halo-2,5-dimethoxy phenethylamines reacted with the Marquis reagent to a give deep yellow-green and/or a deep green color. …
NAID 110006687012

Simple method of detection of the strains of fluorescent Pseudomonas spp. producing antibiotics, pyrrolnitrin and phloroglucinol

Tazawa Junko,Watanabe Kenji,Yoshida Hitoshi [他],Sato Mamoru,Homma Yoshihisa
土と微生物 54(1), 61-67, 2000-04-01
… When the plate was dried out and sprayed with Ehrlich's reagent, a clear red spot appeared at Rf=0.74. … A blue spot was detected on the plate at Rf=0.68 after spraying with Gibbs' reagent. …
NAID 110009468015

Variety of Intracellular Products among Recombinants Obtained by Interspecific Protoplast Fusion in Streptomycetes

Okanishi Masanori,Yamaura Yumiko,Furuta Takaki
Bioscience, biotechnology, and biochemistry 61(4), 748-751, 1997-04-23
… Detection with anisaldehyde-sulfuric acid reagent and Ehrlich's reagent showed that 29-37% of the recombinants produced new products different from those of the parental species, while about 50% of the recombinants did not produce any detectable substances. …
NAID 110002678571

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試薬

関: indicator

[opium-1] Faubert Maunder, MJ. "Field and laboratory test for raw and prepared opium.". Bulletin on narcotics 27 (1): 71–6. PMID 1039285.

[microgram2005-2] Spratley, Trinette (2004). "Analytical Profiles for Five "Designer" Tryptamines" (PDF). Microgram Journal 3 (1-2): 55. Retrieved 2013-10-09.

[3] O’Neal, Carol L; Crouch, Dennis J; Fatah, Alim A (April 2000). "Validation of twelve chemical spot tests for the detection of drugs of abuse". Forensic Science International 109 (3): 189–201. doi:10.1016/S0379-0738(99)00235-2.

[NIJColorTest-4] "Color Test Reagents/Kits for Preliminary Identification of Drugs of Abuse" (PDF). Law Enforcement and Corrections Standards and Testing Program. July 2000. Retrieved 2011-07-24.

[5] 02 July 2014. "Ehrlich's Reagent Safety Data Sheet" (PDF). Labchem. Retrieved 11 January 2015.

[6] Ehmann, A. (1977). "The van URK-Salkowski reagent — a sensitive and specific chromogenic reagent for silica gel thin-layer chromatographic detection and identification of indole derivatives" (PDF). Journal of Chromatography A 132 (2): 267–201. doi:10.1016/S0021-9673(00)89300-0. edit

[7] Maunder, M. J. de Faubert (August 1974). "A field test for hallucinogens: further improvements". Journal of Pharmacy and Pharmacology 26 (8): 637–638. doi:10.1111/j.2042-7158.1974.tb10677.x.

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Ehrlich reagent