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Non-viral transfection methods optimized for gene delivery to a lung cancer cell line.

Salimzadeh L, Jaberipour M, Hosseini A, Ghaderi A.SourceShiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran.
Avicenna journal of medical biotechnology.Avicenna J Med Biotechnol.2013 Apr;5(2):68-77.
BACKGROUND: Mehr-80 is a newly established adherent human large cell lung cancer cell line that has not been transfected until now. This study aims to define the optimal transfection conditions and effects of some critical elements for enhancing gene delivery to this cell line by utilizing different
PMID 23799175

Optimization of the transductional efficiency of lentiviral vectors: effect of sera and polycations.

Denning W, Das S, Guo S, Xu J, Kappes JC, Hel Z.SourceDepartment of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294-2182, USA.
Molecular biotechnology.Mol Biotechnol.2013 Mar;53(3):308-14. doi: 10.1007/s12033-012-9528-5.
Lentiviral vectors are widely used as effective gene-delivery vehicles. Optimization of the conditions for efficient lentiviral transduction is of a high importance for a variety of research applications. Presence of positively charged polycations reduces the electrostatic repulsion forces between a
PMID 22407723

Biochemical properties and comparative pharmacology of a coagulant from Deinagkistrodon acutus snake venom.

Tang SS, Wang XH, Zhang JH, Tang BS, Qian L, Li PY, Luo LW.SourceGuangdong Key Laboratory of Pharmaceutical Bioactive Substances, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou 510006, China. Electronic address: songstang@gmail.com.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences.Eur J Pharm Sci.2013 Feb 18;49(1):90-98. doi: 10.1016/j.ejps.2013.02.002. [Epub ahead of print]
A number of snake venom thrombin-like enzymes (TLEs) have already been characterized. Some TLEs play significant roles in vessel injury hemostasis. A novel TLE (Agacutase) was purified from Deinagkistrodon acutus snake venom by the means of Sephadex G-75, DEAE-Sepharose FF, and Sephadex G-25 column
PMID 23429184

江下優樹,東原絢子,大西政康,水野正明,吉田純,住吉秀明,吉岡秀克,高崎智彦,石川雄一,久保田直治,大西靖彦,エシタユウキ,ヒガシハラジュンコ,オオニシマサヤス,ミズノマサアキ,ヨシダジュン,スミヨシヒデアキ,ヨシオカヒデカツ,タカサキトシヒコ,イシカワユウイチ,クボタナオジ,オオニシヤスヒコ,Eshita Yuki,Higashihara Junko,Onishi Masayasu,Mizuno Masaaki,Yoshida Jun,Sumiyoshi Hideaki,Yoshioka Hidekatsu,Takasaki Toshihiko,Ishikawa Yuichi,Kubota Naoji,Onishi Yasuhiko
大分大学工学部研究報告 57, 1-8, 2009
… Transfection efficiency of 2-diethyl aminoethyl-dextran-methyl methacrylate graft copolymer (DDMC) was evaluated with pGL3 DNA and COS-7 cells. … Results were as follows: 1) When DNA (0.05 ㎍/well) and DDMC (4.0 ㎍/well) were transfected into the cells, RLU was about double higher than DNA and DEAE-dextran. … 2) The first peak of RLU on DDMC and DEAE-dextran appeared at 72 hours and 48 hours after the transfection, respectively. …
NAID 120002187927